Traditional Silver staining is not compatible to mass spectrometric analysis, but several modified silver staining procedures which eliminate the use of glutaraldehyde- and formaldehyde- based sensitizer claim to be more compatible to MS analysis. There are several commercially available silver staining kits which all claim to be MS compatible. However, based on our experience, the yield of peptide from in-gel digestion of a “MS compatible” silver stained gel band is still much lower than that from a colloidal Coomassie stained gel band with same amount of protein. Although we do accept silver stained gel samples, we encourage our customers to make effort to purify enough protein sample for using colloidal Coomassie, Cu or Zn staining (its detection limit around 10 ng), and use silver staining when purifying more protein is too difficult. The effort to optimize a purification procedure are often paid back greatly in a long run since it often lead to high success rate in identification of these protein at high confidence level, and such experience is important to future work.