| Since each step in our protein identification has been
optimized and strictly controlled, each sample should meet following
requirement in order to achieve the best results.
Requirement for Gel Samples:
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The height of each gel slice (vertical dimension) should be less
than 2.5 mm. A gel slice higher than 2.5 mm will be trimmed to 2.5 mm
in our processing.
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Each 1D gel sample should contain only one visible protein band.
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For a sample with low amount, you may send us a duplicate sample in
a separate tube as a backup. There is no extra charge.
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Please send us a copy of a photograph of uncut gel with submitted
protein bands (spots) clearly marked. Such a photograph may help us to
decide the best procedure for analyzing these samples.
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In the case that a silver staining are needed in order to visualize
protein bands, one should use a MS-compatible silver staining protocol
or kit (such as Invitrogen SilveQuest).
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Each sample should be free of contamination. Direct contact with
bare hands, dirty gel containers, or the surface of a light box should
be avoided.
For Solution Samples:
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A solution sample shouldn't contain excess amount of antibodies,
bait protein or other proteins in excess amount. The presence of a
dominant protein in a solution sample often prevents the
identification of many other less abundant proteins. Running a SDS-PAGE
and identifying proteins with gel samples is often a better
alternative in such a case.
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Use a small potion of a solution samples to run a SDS-PAGE to
confirm its quality before shipping sample to us (to avoid wasting
money on a bad sample). Please send us a copy of the gel photograph
together with your sample.
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All solution samples must be shipped in a dry ice container.
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