The height of each gel slice (vertical dimension) should be less
than 2.5 mm. A gel slice higher than 2.5 mm will be trimmed
automatically to 2.5 mm
in our processing. Although we will make our best effort to leave
the major protein band, we cannot guarantee the protein band left
after the trimming is the band of your interest.
Each 1D gel sample should contain only one visible protein band.
For a sample with low amount, you may send us a duplicate sample in
a separate tube as a backup. There is no extra charge.
Please send us a copy of a photograph of uncut gel with submitted
protein bands (spots) clearly marked. Such a photograph may help us to
decide the best procedure for analyzing these samples.
In the case that a silver staining are needed in order to visualize
protein bands, one should use a MS-compatible silver staining protocol
or kit (such as Invitrogen SilverQuest).
Each sample should be free of contamination. Direct contact with
bare hands, dirty gel containers, or the surface of a light box should
be avoided.
