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| Advantages of Our ProteinID Service |
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- Ultra high sensitivity
By using a 75 um inner diameter capillary column in LC-MS, the
detection sensitivity can theoretically increase 3762 folds
compared to a 4.6 mm column LC-SM system ((4.6mm/0.075mm )2=3762).
Our detection sensitivity is further increased by using an ion
trap mass spectrometer, which has the capacity to accumulate ions
over a chromatographic peak, and is still the most sensitive
instrument to date. The use of manual in-gel digestion also
contributes to our ultra high sensitivity. Various studies have
indicated that protein identification with manual in-gel digestion
is 5-10x more sensitive than that with robotic digestion.
- Reliability
All proteins reported by our ProteinID service are definite
identifications. No matter how many proteins are identified in a
sample, each reported protein should have better than with
>99.99 possibility if two or more peptides are sequenced or
>99% if only one peptide is sequenced. In contrast, MALDI-TOF
based protein identification only reports a candidates lists with
a probability score associated each candidate, and only one out of
those candidates may be the protein present in a sample. There is
no guarantee that the proteins in the sample have the highest
probability score or even presence in the candidate list.
- Ability to Identify Protein Mixture
We often identify 3-10 proteins in a single 1D gel band, with
the minor proteins only 1/10 to 1/20 of the major ones. And we
often identify 10-30 proteins from a solution sample. In contrast,
based on the experience of others, and ours MALDI-TOF based
protein identification is generally incapable of analyzing a
sample with more than two proteins. MALDI-TOF-TOF based protein
identification fails to identify some lower level proteins in such
a sample.
- Guaranteed Success
We guarantee a successful identification for each eligible
samples or it will be free of charge.
- A Strong Track Record
We have provided our proteinID service to a large number of researchers
from all major US universities and many US government research
institutions, as well as researchers from Europe, Asia, and Latin
America. The high quality of our protein ID service is
demonstrated by a large number of recent publications in which
authors have kindly cited our work in their publications.
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