The complete service package includes cDNA
subcloning into an expression vector, confirmation of a expression
construct by DNA sequencing, colony construction, optimization, small
scale expression, optimization of expression and purification
conditions, expression of proteins from 1 to 20 liters cell culture,
purification of the expressed protein by various chromatographic
techniques, confirmation of the identity and purity of the expressed
protein by SDS-PAGE, protein identification and protein peptide
mapping. Optionally, in the case where a biochemical activity assay is
available for the protein, we may also carry out such an assay.
By default, an expressed protein will have a FLAG tag
at its N- or C- terminus with the use of our in-house developed
expression vectors. We also supply monoclonal anti-FALG antibody that
can be used for immunoprecipitation, western blot and other assays
with a very low background. We also provide the option to remove the
FLAG tag from expressed protein products, as well as to separate the
cleaved FLAG tag peptides from the delivered protein products.