It is often a very difficult task to isolate the in vivo molecular targets of a drug molecule, since such isolation often completely depends on moderate interactions between the drug molecule and its target proteins. Inside cellular environment where many other specific and non-specific interactions are present, the interactions between a drug molecule and its specific targets are often not strong enough to provide enough folds of purification to isolate specific target proteins.

To overcome this difficulty, we have developed a novel technology (patent pending) that is capable of specifically identifying target proteins even in the case where hundreds of contamination proteins are co-purified with low abundant target proteins.

To identify the in vivo targets of a drug molecule, a customer needs to provide us the drug compound (or its analogs) that contains at least one active site in its chemical structure (e.g. a primary amine group, a sulfhydryl group or a carbonyl group), so we can immobilize the drug molecule onto the surface of solid matrix to make a specific drug affinity column. A protein lysate made from relevant cells or cellular organelles also need to be provided by our customers or purchased from a commercial source. We’ll carry out affinity chromatography purification, isotope labeling, LC-MS and LC-MS/MS analysis. The data will then be analyzed by ProtTech’s Target ID software and fully validated by our senior scientists before reporting.

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