Colony selection is an important step in therapeutic protein discovery. Finding an optimized strain that can produce an active and suitable biological product with high yield can significantly impact large-scale production and downstream processes. We have developed a LC-MS/MS based method that can rapidly and quantitatively screen colonies for producing designed protein forms with high yield. This method can complement the limitations of conventional colony selection methods such as western blot, ELISA and enzymatic assays, and offers significant advantage over those methods in the case where immunological or enzymatic assays are not available or difficult to carry out.

• Protein Mixture Quantitation & Impurity Analysis

• Peptide Mapping

• Disulfide Bond Analysis

• Glycosylation Analysis

• Intact Molecular Weight Measurement

• N-terminal and C-terminal Sequencing

• Electrophoresis and Isoelectric Focusing Analysis

• Liquid Chromatographic Analysis

• Antibody Epitope Mapping

• Identifying Antibody Molecular Targets

• De Novo Sequencing of mAb

• Software for Characterizing Therapeutic Proteins

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