Epitope mapping is an important tool in the discovery of high affinity and high potency therapeutic antibodies. ProtTech provides a customized service for epitope mapping.

Although the method of screening peptide library has been used widely in epitope mapping, it has only achieved limited success. This is because, in about 90% of cases, the antigenic determinant bound to an antibody is not a linear stretch of amino acids but a uniquely folded tertiary structure, which makes the epitope mapping a difficult task.

We have developed an epitope-mapping platform that can protect the epitope regions of an antibody-antigen complex during limited proteolytic digestion. We use a combination of specific and non-specific proteolytic enzymes such as trypsin, Glu-c, pepsin, chymotrypsin to digest antibody-antigen complexes under a well-controlled condition. The resulted peptides from each digest are analyzed by LC-MS/MS, and the protection patterns are then analyzed by our ProtTechEpiMap software to define the peptide regions that bound to antibodies.

• Protein Mixture Quantitation & Impurity Analysis

• Peptide Mapping

• Disulfide Bond Analysis

• Glycosylation Analysis

• Intact Molecular Weight Measurement

• N-terminal and C-terminal Sequencing

• Electrophoresis and Isoelectric Focusing Analysis

• Liquid Chromatographic Analysis

• Colony Selection

• Identifying Antibody Molecular Targets

• De Novo Sequencing of mAb

• Software for Characterizing Therapeutic Proteins

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